In an ELISA, the secondary antibody-enzyme conjugate recognizes and binds to the primary antibody.    

Select the appropriate words that will complete the following sentence respectively. During the annealing step of a PCR reaction, a short piece of nucleic acid called —————-binds to the template DNA  with —————–

In this quiz, you should be able to access our digital textbook through this link, also here: https://connect.mheducation.com/class/t-matthies-spch-1318-n02-spring-2025 *You may need to type your login information for McGrawHill Connect.  If so, this may not be the same as your Blinn login.  This will be the email address and password you originally used to set up your McGrawHill Connect account. For this quiz question, please answer the following: 1 – Were you able to access the textbook? YES OR NO 2 – If Yes, please type YES! & Did you have to log into McGrawHill or did the Connect screen simply pop-up? 3 – If No, please tell me more.

The picture below shows an immunodiffusion assay done to detect the presence of antibodies to Influenza virus in a sample obtained from a patient.  Peripheral wells 1 through 6 contain various dilutions of the patient sample.  What should be placed in the central well 7?

A bacterial colony represents

You are determining the purity of water sample by plating a small volume on a media that selectively allows the growth of coliforms.  A colony that would grow on this media would appear ——— color upon gram staining.

You want to make 50 mL of a 1:100 dilution of a bacterial sample.  How many milliliters of the bacterial sample should be transferred into water in order to achieve this dilution? (Remember, you want a final total volume of 50 mL).

  —————- cells have a thin layer of peptidoglycan, while ————— cells have a thick layer of peptidoglycan in their cell walls.

Shigella is an enteric pathogen that is associated with severe, bloody diarrheal disease.  Which of the following tests will differentiate Shigella from E.coli as the causative agent of disease? You may use the chart below as a reference to decide your answer.

You are given a sample of bacteria at a concentration of 3 x 106 CFU/mL.  After mixing the sample , you transfer 1 mL into 99 mL of fresh media and label this Tube 1.  You mix the contents of Tube 1 and transfer 1 mL into 9 mL of fresh media and label this Tube 2.  Based on this dilution scheme, the estimated CFU/mL of  bacteria in Tube 2 should be: