You use Sanger sequencing on a 1000 bp insert, but after ~500 bp the data becomes difficult to interpret. What is the next step?

Which of the following will occur if the annealing temperature in PCR is 5 °C below the primer Tm?

In RAPD PCR,

Who discovered Thermus aquaticus, the bacteria from Yellowstone which was the source of Taq polymerase?

You want to design a protein that contains an EcoRI cut site on the N-terminus, and a BamHI site at the C-terminus. What should the primers look like?

A technique that involves eliminating genomic DNA, using reverse transcriptase to generate cDNA, and using a hot-start polymerase and SYBR Green dye is

A VNTR analysis is performed on a mother, father, and child at a specific locus, shown below. Which of the following is TRUE?

You want to clone a 5000 bp piece of DNA. What is the best way to accomplish this?

In asymmetric PCR,

DNA can be detected in an agarose gel by using each of the following EXCEPT