Which technique was used to sequence the human genome via shotgun sequencing?

Which of the following PCR techniques involves incrementally lowering the annealing temperature in each subsequent cycle?

Which of the following is TRUE about DNA purification from an agarose gel?

You insert a gene into the vector below using the HindIII and PstI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

Which of the following PCR techniques will produce a single-stranded product?

You wish to purify His-tagged β-actin, starting from a human cell line. The first step of this process would be to

Isothermal amplification, which can detect pathogens at room temperature, uses

You insert a gene into the vector below using the HindIII and PstI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

You run a PCR product on a gel and see multiple nonspecific bands, and smearing. Which of the following is NOT a likely cause?

Which of the following techniques involves using a primer to sequence a fragment of DNA and then using this new sequence as a primer to sequence the next stretch of DNA?