Which is NOT an advantage of ligation independent cloning?

In the 1970s, researchers could use each of the following tools to study DNA EXCEPT

In bisulfite sequencing,

Which DNA purification technique involves purifying the sample using an ultracentrifuge?

You are attempting to incorporate a mutation in a DNA sequence using PCR. To accomplish this, you design four primers. Which of the following primers would you use during PCR #2 shown in the image below?

Bisulfite sequencing

Which of the following proteins was patented by comparing it to phosphorylase B and bovine serum albumin on an acrylamide gel?

Which of the following is NOT required in Maxam-Gilbert sequencing?

In the pedigree below, which represents the dot blot you’d expect of the parents?

Why are cloning vectors preferred to expression vectors for initial cloning of a gene?