Wacc

15.  Nitrate reduction test can be used to differentiate between Pseudomonads and the Enterobacteriaceae.  You innoculated your Nitrate broth with your unknown; after incubation you added 5 drops of reagent A and 5 drops of reagent B.  You observed a Red color within 2 minutes.  Is this a + or a – reaction?

20.  Name 2 enzymes responsible for the breakdown of Starch to D-glucose.

19.  Starch exists in 2 forms, name them.

How does a high calorie, high protein diet help improve complications of the liver?

23.  These are Lysine decarboxylase media.  Describe the reactions in Tubes A and C only.  After inoculation, we added a reagent to the inoculated tubes , what was that reagent and what was the purpose?

3. You performed an Elisa test in the lab to detect antibody against HIV in the patient’s serum.  The test was found to be 96% sensitive, what does that mean? 

12.  You were able to identify it as Streptococcus.  What is the next thing you would do to speciate it (i.e. into species)?

21.  You are showing a high school student who is shadowing you (following you around) in your lab class, a Light Compound Microscope. Name 3 things you will tell him/her that will help the student to recognize the oil immersion objective lens.

Compare and Contrast (give 1 similarity and 1 difference) between DNA polymerase and Reverse Transcriptase.