A 39-year-old patient is being seen in the clinic for evalua…

A 39-year-old patient is being seen in the clinic for evaluation of osteoarthritis. The patient is 281 lbs and 5’9″. The patient has a past medical history of hypertension, sleep apnea, and hyperlipidemia. Vital signs:  Blood pressure 140/92 mmHg Heart rate: 88 bpm Respiratory rate: 18 breaths per minute Oxygen saturation: 94% on room air Medications include: Lisinopril 20mg every evening Atorvastatin 40 mg daily Ibuprofen 400 mg PRN pain Labs are drawn which include the following: Test Result Normal Range Total cholesterol 230 mg/dL < 200 mg/dL HDL 40 mg/dL > 50 mg/dL LDL 150 mg/dL < 100 mg/dL Triglycerides 180 mg/dL < 150 mg/dL Fasting blood glucose 145 mg/dL 80 - 100 mg/dL HgA1c 8.5% < 5.7 % ALT 45 U/L 4  -36 U/L Based on the history and recent labs, the nurse anticipates that be added due to and that be adjusted due to .

You are studying an enhancer that you have found is usually…

You are studying an enhancer that you have found is usually acetylated in early embryos. You wish to test whether this acetylation is relevant for its function by causing it to be deacetylated and measuring the transcription rates of nearby genes. Which of the following approaches could test this?