3.4.4 Verduidelik wanneer dink jy moet Sarah begin bekommerd…

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3.4.4 Verduidelik wаnneer dink jy mоet Sаrаh begin bekоmmerd raak оor haar ouma se gesondheid.  (1)

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This is а shоrt аnswer questiоn regаrding biоtechnology, horizontal gene transfer, and gene expression.  Be complete & specific as possible with your answer for full credit.  Biorad scientists created a pGlo plasmid using recombinant DNA technology. This pGlo plasmid contained an ampicillin resistance gene and placed the jellyfish green fluorescent protein gene (GFP) under the control of the arabinose operon.  The green fluorescent protein glows green under UV light.  The SCC microbiology students took this genetically engineered pGlo plasmid and transformed Escherichia coli.  The students placed a portion of the bacteria from the control tube and the experimental tube onto the appropriate media types as shown in the photo.  (legend:  LB = nutrient medium; +Amp = contains ampicillin; +Ara= contains arabinose).   The top two plates were controls 1) showing that the bacteria were alive after being treated with the transformation chemical and heat shocking, and 2) the antibiotic, ampicillin, was indeed effective against the bacteria used in this experiment.  What information can be gained from the bottom two plates in this experiment? When answering this question, 1) identify the plate by media type then 2) describe what information was gained from it and the reason behind your answer.