Suppose you have 150 ng of 1.5 kb vector (not dephosphorylat…
Suppose you have 150 ng of 1.5 kb vector (not dephosphorylated). How much 0.5 kb insert should you use for the ligation reaction?
jwt-auth domain was triggered too early. This is usually an indicator for some code in the plugin or theme running too early. Translations should be loaded at the init action or later. Please see Debugging in WordPress for more information. (This message was added in version 6.7.0.) in /home/forge/wikicram.com/wp-includes/functions.php on line 6121wck domain was triggered too early. This is usually an indicator for some code in the plugin or theme running too early. Translations should be loaded at the init action or later. Please see Debugging in WordPress for more information. (This message was added in version 6.7.0.) in /home/forge/wikicram.com/wp-includes/functions.php on line 6121