Below are the results of a series of experiments on fruit fl…
Below are the results of a series of experiments on fruit flies with two different phenotypes. Flies with phenotype 1 can digest rotting fruit without any problem while those with phenotype 2 have a drunken appearance and sometimes even die from eating rotting fruit. The researchers hypothesize that the two phenotypes are the result of differences in the enzyme alcohol dehydrogenase (Adh), which catabolizes the ethanol produced when fruits rot. The researchers decide to do three tests of this hypothesis. First, they do a northern blot for Adhin individuals of both phenotypes. Second, they do a western blot for Adhin individuals of both phenotypes. Finally, they sequence a portion of the 5’ end of the coding strand of the DNA of individuals of both phenotypes. The results of each are shown below. (A.) Interpret the results of the northern and western blots. (B.) Determine the coding sequences of the alleles of both phenotypes (label 5’ and 3’ ends). (C.) What do you hypothesize is the cause of the different phenotypes? (D.) What data would you need to generate to test your hypothesis?(12 points)