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Use the infоrmаtiоn prоvided in the CASE below аlong with the аttached Ab Screen and ABID panel antigrams to answer questions A-D. Please answer the questions in the order presented, using letters provided to identify corresponding responses. CASE: Maria Giovanni is a 36-year-old female who has been in a motor vehicle accident (MVA). She has been transported to the local ER and must have surgery for a compound fracture in her left leg. The ER physician has ordered 4 units of RBCs to be crossmatched (XM) for surgery. Her medical record indicates that she has had three previous pregnancies, and received one unit of RBCs during her last delivery two years ago. Initial Blood Bank Results Blood Group Type: O D Positive (Verified by repeat testing)Antibody detection (Screen): Positive MLS327 FINAL EXAM ABID Problem Maria Giovanni-2024.docx Students are to use the following guidelines to rule-out clinically significant antibodies. Antibodies against blood group antigens known to demonstrate dosage must be ruled-out using a homozygous screen or panel cell. These include the following blood groups: Duffy (Fya/Fyb) Kidd (Jka/Jkb) MNS (MN /Ss) All other antibodies can be ruled-out using one homozygous or two heterozygous reagent RBCs (using cells on either the Ab screen and panels, including selected cell panels.) QUESTIONS Using guidelines established in this course for ruling-out and Rule of 3 for antibody identification, identify the most likely antibodies in the patient's plasma. (8 points) List all other antibodies that cannot be ruled out. (2 point) Explain the variation in the strength of reactivity observed with different panel cells. (2 points) Is the antibody considered clinically significant? Support your answer using results for ABID work-up. (2 points) Analyze the patient's medical history and describe how the patient developed the antibody that you identified? Include all possibilities, if there is more than one. (2 points) What transfusion recommendation would be indicated in the patient's medical record to provide suitable RBC for safe transfusions in the future? (4 points)