A DNA fragment of unknown sequence is divided into four port…
A DNA fragment of unknown sequence is divided into four portions and mixed with all the elements necessary to synthesize the complementary strand according to the Sanger sequencing method. To each of these four mixtures a different dideoxynucleotide (a modified base) is added in addition to the normal nucleotides. Each mixture is then separated by electrophoresis and the gel shown below is obtained. What is the sequence of the original strand of DNA? Note: The A, T, C, G labels at the top of each lane refer to the dideoxynucleotide that was added in each reaction mixture.