A primer used in dideoxy DNA sequencing is 20 nucleotides lo…
A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following sequence: 5’-GGATCCATGACTAGTCCGAC-3’. A segment of DNA is cloned into a vector and then the vector DNA is denatured and subjected to dideoxy DNA sequencing method. Below is the DNA sequence from a region of the vector. The primer-annealing site is shown in bold and underlined. 3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACT-5’ Based on the sequence above, what would be the size(s) of the band(s) (i.e., the number of nucleotides in each band) in which dideoxyT had been added to the sequencing reaction?