Three different PCR reactions using a primer set with a pe…
Three different PCR reactions using a primer set with a perfect Tm of 50 C were performed. The expected product of each PCR was 1000 bp. The products obtained in each separate reaction were analyzed by electrophoresis. The results observed in the gel are depicted in the Figure. Which of the thermocycler programs described below was more likely used to obtain the results observed in Line 2; Line 3 and Line 4. The line 1 correspond to the molecular weight marker.