You are working in the microbiology laboratory and received…
You are working in the microbiology laboratory and received an order for an Aerobic Culture, Sputum. You review the patient information and determine that the patient is a 10 year old male with cystic fibrosis (CF). You plate the sample on blood (BAP), chocolate (CHOC) and MacConkey (MAC) agar. The mucoid-appearing organism on BAP has a greenish, metallic sheen, flattened colony morphology and spreads out over the agar surface. CHOC agar plates contain a large, gray organism with a mucoid morphology, in addition to normal oropharyngeal flora. The MAC agar plates contain a clear, mucoid, non-lactose fermenter (image below). Colony gram stain of the BAP, MAC, and CHOC colonies all show a Gram-negative rod. Triple sugar iron agar testing reveals a glucose and lactose non-fermenter (TSI K/K; alkaline slant and butt) that is negative for gas and hydrogen sulfide production. What is the most likely organism? Organism on MacConkey Agar