When measuring DNA concentration using spectrophotometry, th…
When measuring DNA concentration using spectrophotometry, the purity of the DNA is also often tested to ensure that there is minimal contamination with other biomolecules. The most prominent test of DNA purity accounts for the possible presence of contaminating proteins, aromatic amino acids in particular. What ratio of absorbances (A) at 2 different wavelengths is most commonly used to determine DNA purity?