Antibody & TCR Diversity (A) Diversification of the T cell…
Antibody & TCR Diversity (A) Diversification of the T cell receptor (TCR) primary repertoire is generated using the same mechanisms as for immunoglobulin genes. Briefly define each of the four genetic mechanisms involved in generating Ab and TCR diversity: Germline; Combinatorial; Junctional; and Association. (4 points) (B) Why is it that the junctional diversity produced by addition of “P” and “N” nucleotides occurs in the CDR3 region and not the other two CDRs? (2 points) (C) Briefly define why a VH segment cannot join directly with a JH segment in heavy-chain gene rearrangement. (2 points) (D) Briefly define one advantage and one disadvantage of junctional diversity during the rearrangement of immunoglobulin gene segments. (2 points)