Below is shown a simplified hypothetical organism, eggman. Y…
Below is shown a simplified hypothetical organism, eggman. You are researching gene1, and intend to create a transgenic eggman line that constitutively overexpresses gene1 across all regions of its body throughout development. Given the scheme below, describe the smallest/simplest transgene that could be added to the eggman genome to accomplish expression of gene1 throughout the entire body of eggman. Your answer should interfere with other genes as little as possible, and your answer does not need to name components related to genomic integration methods, only the functional transgene locus that could drive gene1 expression throughout eggman. Reminder about how eggman works: four transcription factors (magenta, yellow, green, and blue) are all maternally deposited in the pattern shown in the drawing on the left. Those transcription factors (TFs) bind enhancers corresponding to their color (“E” boxes) and drive expression of the head, arm, and leg genes. In this version of eggman, we also have a black transcription factor that is driven by all four maternally deposited TFs, as well as itself. The black TF also drives the expression of an important housekeeping gene that is required for cell survival in eggman.