You are the technical supervisor of the blood bank.  One of your techs suggests that it would be more cost effective to perform antibody screening on patients using commercially available pooled reagent red blood cells rather than a three-cell screen.  The tech reasons that the pooled reagent requires only one gel well for testing versus three wells for the typical screen cells.  This would mean cost savings!  Is it acceptable to use pooled screening cells for patient pre-transfusion testing?  Why or why not?

What results would be expected if the same panel were retested after enzyme treatment?

A type and screen is done on a 49-year-old woman who is scheduled for a hysterectomy in 1 week. Her blood type is A-positive, and her antibody screen was positive. What must be done before her surgery date?

A male patient was seen in the emergency room with an acute bleed. The recommendation from the blood supplier is to give O-positive RBCs as uncrossmatched blood. This patient has already been exposed to Rh-positive blood after a previous accident. What is a possible outcome?

A 28-year old male police officer was brought to the emergency room for gunshot wounds and 4 units of RBCs were ordered for emergency surgery.  The following results were obtained:   ABO Grouping Rh Typing Weak D Testing     Forward Reverse Patient cells with AHG CC   Patient cells with Patient plasma with   Anti-A Anti-B Anti-A,B A1 B A2 Anti-D D Control Weak D Weak D Control Weak D Weak D Control   Patient 00123 0 0   4+ 3+   very weak 0 1+ 0   √   The antibody screen is positive and the technician proceeds with a panel.  The panel conclusion is the presence of anti-D antibody but anti-E cannot be ruled out. The AABB standards state that persons who test as weak D positive may receive Rh positive blood. In this case, would the policy be honored? Identify how the technician should prepare units for transfusion.   Explain the ABO/Rh and antibody panel results. Provide justification for your decision and next steps.

A positive autocontrol in antibody detection procedures is usually indicative of:

A type-A person demonstrates a 3+ reactivity with A1 cells (reverse grouping). The forward grouping with anti-A is 4+. Therefore, the possibility of a subgroup is excluded. B cells demonstrate a 4+ reaction. The antibody screen is weakly positive at 37°C, but shows no reactivity at the AHG phase. An antibody panel is performed and anti-M is identified. The patient phenotypes negative for M antigen. How is the ABO discrepancy resolved?

Which of the following reagents or methods is best for categorizing partial D types?

Given the following results, what is the probable cause of a positive reaction in the major crossmatch? IS = 0   37°C = 0   AHG = 2+   CC = ND   Auto-control was negative

In performing an antibody screen by the solid phase technique, a monolayer of red blood cells is formed at the top of the microplate wells following the addition of indicator cells. This result should be interpreted as: