You inoculate a flask containing tryptic soy broth with a cu…

You inoculate a flask containing tryptic soy broth with a culture of E. coli and begin measuring the optical density of the culture with a spectrophotometer in order to detect growth. The measurements you take for the first few hours do not show any change, and then later in the day you begin to observe increases in numbers of cells (i.e., your absorbance readings increase). Which of the following best explains why there is this delay in growth following inoculation?

Optional up to 2 points of extra credit: Here we see an esti…

Optional up to 2 points of extra credit: Here we see an estimation of daily infections by SARS-CoV-2 based on wastewater data collected during the COVID pandemic, tracking the repeated waves of infection we have experienced in the U.S. 1. How is it that we are able to measure SARS-CoV-2 in wastewater, i.e., what does this tell us about infections with this virus? What is it that we are measuring in this wastewater? 2. Make a prediction based on this graph and your understanding of the ongoing COVID pandemic. Where do you think cases will go in the future? Explain your thinking – why do you think cases will behave as you predict, and what variables will contribute to that trend.

Clostridium perfringens, causative agent of gas gangrene (re…

Clostridium perfringens, causative agent of gas gangrene (resulting e.g. from shrapnel wounds) has an extraordinary doubling time of only about 8 to 10 minutes when in the human host. If a patient is brought in with a shrapnel wound with fluids containing an average concentration of about 100 C. perfringens cells per ml, assuming a doubling time of 10 minutes, about what concentration of cells will the patient have in the wound in about an hour if left untreated for this time?

You sample some surfaces using a sterile cotton swab moisten…

You sample some surfaces using a sterile cotton swab moistened with sterile water, and streak the swab onto the surface of two different types of media: one Petri dish containing the complex medium tryptic soy agar (TSA), and the other the chemically defined medium glucose salts agar (GSA). Following incubation, on which Petri dish do you expect more growth, and why? Please briefly explain, and be sure to compare and contrast these two types of media as part of your answer.