Upload your answers to these questions. Beginning with PLP…

Upload your answers to these questions. Beginning with PLP (Vit. B6), whose structure is given below, draw out the arrow-pushing mechanism that results in the transfer of the amino group of Ala to PLP as catalyzed by the enzyme alanine aminotransferase. (4 pts.)  Name the molecule that ultimately accepts the amino group of Ala in the above alanine aminotransferase reaction. (1 pt.)  The α-amino group in the amino acid glutamate can be directly converted to an α-keto compound in a single step with the release of ammonia. This reaction requires a cofactor. Identify the cofactor and the general name for the type of enzyme that catalayzes reactions like these. (2 pts.) 

Upload your answers to these questions. In gluconeogenesis,…

Upload your answers to these questions. In gluconeogenesis, oxaloacetate can be converted in a single step in the matrix of the mitochondria to an intermediate, which is not the final, but the penultimate (next to last) compound in glycolysis. Show this reaction. Draw the structure and name(s) of the product(s) of this reaction and give the name(s) or acceptable abbreviation(s) of any necessary cofactor(s) or co-reactants for the reaction, as well as the name of the enzyme that catalyzes this reaction. (3 pts.) When cytosolic NADH is insufficient to run the GAP dehydrogenase reaction in reverse, oxaloacetate can also be converted to the same penultimate glycolysis intermediate in the cytosol instead of in the matrix. Outline the set of reactions in this case, beginning with oxaloacetate in the mitochondrial matrix and ending with the formation of the penultimate glycolysis intermediate in the cytosol. Structures or names or acceptable abbreviations of compounds and cofactors are sufficient, but all substrates and cofactors must have either a structure or name given. (4 pts.)  Oxaloacetate connects to fatty acid biosynthesis via the reaction that forms the shuttle compound responsible for transferring fatty acid building blocks from the mitochondrial matrix to the cytosol. Draw out the reaction that synthesizes this shuttle compound. Draw the structure and name of the product(s) of the reaction and give the name(s) or acceptable abbreviation(s) of any necessary cofactor(s) or co-reactants for the reaction. (3 pts.) 

Write your answers to the following four questions in the bo…

Write your answers to the following four questions in the box below.  Explain what drives the initial palmitate reaction to tagging the palmitate to completion. (2 pts.) Briefly outline how this prepared fatty acid is transported from the cytosol to the matrix.  (2 pts.) The final enzyme of the series of reactions of β-oxidation that releases acetyl-CoA uses covalent catalysis. What is the nature of the covalent bond between the substrate and this enzyme? (2 pts.) Under fasting conditions in the liver, the level of oxaloacetate falls. Briefly explain why this happens and how this would affect acetyl-CoA levels in the matrix. (2 pts.)