What has the greatest influence on protein stability?

You have purified a new peptide hormone.  To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide.    Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following.    Ser─Leu Asp─Val─Arg Val─Met─Glu─Lys Ser─Gln─Met─His─Lys Ile─Phe─Met─Leu─Cys─Arg   Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation:   His─Lys─Ser─Leu Asp─Val─Arg─Val─Met Glu─Lys─Ile─Phe─Met Leu─Cys─Arg─Ser─Gln─Met   Determine the sequence of the intact protein.

You are trying to separate 2 proteins by gel exclusion chromatography . Listed below is information about each protein.   Molecular Weight pI Protein 1 55 kDa 4.2 Protein 2 22 kDa 8.7 Which protein will elute first from the column? Give a reason for your choice Which other method could you use to separate this proteins? Explain

The difference between a JHA and a JSA is that a JHA is for high hazard industries and a JSA is for “safer” industries. 

Noise is unwanted sound that can be irritating and damaging.

The inner ear responds differently to various frequencies (some frequencies sound louder and some quieter).

This unguarded shaft is…   

According to the OSHA lockout/tagout standard (1910.147)

While physical and atmospheric hazards are considered for the determination of whether or not a space is a permit-required confined space, configuration hazards such as sloping walls, were left out of the confined space standard due to pressure from the agriculture industry which felt that this would place an undue economic burden on the industry.

Full compliance with OSHA noise standards will completely eliminate hearing loss in the workplace.