You have genetically engineered a mutant cell strain that doesn’t produce any functional Talin protein. Congratulations! (Genetic engineering is hard). How do you expect these cells to behave? 

A neutrophil is genetically engineered so that Rac no longer antagonizes Rho. What happens to the cell?

What type of junctions interact with intermediate filaments?  

You are staining cells with a primary antibody that recognizes the FULLY ASSEMBLED death-inducing signaling complex (DISC). Where in the cell do you expect to see the primary antibody concentrated?  (Graphic provided to help visualize the choices below. Blue blob = killer lymphocyte, while the circle is the cell you are staining with the antibody) 

Which steps of cellular respiration occur within mitochondria? (check all that apply)

You learned about how uncoupling proteins (such as uncoupling protein 1, UCP1 or “Thermogenin”) effect mitochondria.  Pharmaceutical companies created diet pills that are “uncoupling agents” that have the same effect as uncoupling proteins on mitochondria. Some of these drugs were found to be lethal and are now outlawed. Based on what you know, what side effects would you expect?

What structures are highlighted in the diagram? 

Microtubules are always assembled with ____________ facing towards the plus end

A mitochondrion is carried by a kinesin motor protein 100’s of nanometers to the opposite end of a cell. This is an example of:

You saw a video in which a researcher conducted a series of experiments to understand how a mutation affecting yeast mitochondria functioned. In the first experiment, he was seeking to determine whether the mutation (present in the -MPC yeast) 1) prevented pyruvate from getting inside the mitochondrion -or- 2) prevented the mitochondrion from breaking down pyruvate into Acetyl CoA properly. To test his hypothesis that the mutation had an effect on pyruvate’s ability to get inside the mitochondrion, he exposed normal yeast and mutant yeast to pyruvate for a period of them, before isolating and lysing the mitochondria of each set, and testing their mitochondrial extracts for levels of these products. If he had gotten the following result indicated in this image, what would this indicate?