Pretend you are scientist studying neurons that are growing…

Pretend you are scientist studying neurons that are growing in a culture dish. You use Fura-2 to measure the intracellular calcium concentration of these neurons. You measure the fluorescence intensity at Time 1 and obtain a ratio indicative of the red trace. Next, you puff a chemical onto the neurons. Immediately after the puff, at Time 2, you again measure the fluorescence intensity. At Time 2 you obtain a ratio indicative of the blue trace. How did the chemical puff affect the neurons? 

The figure below is a modified figure from the paper, “Optog…

The figure below is a modified figure from the paper, “Optogenetics enables functional analysis of human embryonic stem cell-derived grafts in a Parkinson’s disease model”. Which two bars in the figure denote the behavior of “diseased” mice before dopamine cell transplantation?