You are trying to separate 2 proteins by gel exclusion chrom…

You are trying to separate 2 proteins by gel exclusion chromatography . Listed below is information about each protein.   Molecular Weight pI Protein 1 55 kDa 4.2 Protein 2 22 kDa 8.7 Which protein will elute first from the column? Give a reason for your choice Which other method could you use to separate this proteins? Explain

You have purified a new peptide hormone.  To determine its a…

You have purified a new peptide hormone.  To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide.    Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following.    Ser─Leu Asp─Val─Arg Val─Met─Glu─Lys Ser─Gln─Met─His─Lys Ile─Phe─Met─Leu─Cys─Arg   Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation:   His─Lys─Ser─Leu Asp─Val─Arg─Val─Met Glu─Lys─Ile─Phe─Met Leu─Cys─Arg─Ser─Gln─Met   Determine the sequence of the intact protein.