You discover a new regulatory pathway that modulates a fungal defense mechanism in an obscure bacterial group. Given the roles of the different RNAs and proteins described below, select the most logical outcome of each condition or manipulation. Funk1 (Fungus killer 1) is a secreted defense peptide that causes rapid cell death in Penicilium fungi (who secrete penicillin). miRNA2 is a micro RNA that facilitates Funk1 translation by base pairing within the 5′ untranslated region, preventing the formation of a hairpin that otherwise blocks the Shine-Dalgarno sequence. Fureg (Funk1 regulator) is a protein that binds miRNA2 in the absence of penicillin molecules. If penicillin is present in trace amounts and binds Fureg, this changes Fureg conformation such that it releases miRNA2. Penicillin is an antibiotic that is naturally produced and secreted by fungi in the genus Penicilium. (They secrete it to kill bacteria because they compete with bacteria for resources).  Fureg proteins, Funk1 mRNA, and miRNA2 molecules are all constitutively expressed and are normally present in equal numbers in these bacterial cells. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A mutation is introduced into miRNA2 such that it can no longer bind Funk1 mRNA. This should result in . A mutation is introduced into the Funk1 gene such that the Shine-Dalgarno sequence is in-tact, but the 5′ UTR hairpin can no longer form. This should result in . Experimental overexpression (100x dose) of miRNA2 should result in . A mutation is introduced to the gene encoding Fureg such that it can no longer bind miRNA2 in any condition. This should result in .

Eukaryotic mRNAs usually get modified by the addition of a guanine nucleotide that is thereafter modified with an extra methyl group (making 7-methylGuanosine), referred to as a “cap.” This guanine nucleotide is added to the side of the mRNA by the enzyme , which happens transcription. 

This group’s anti-Communist stance led them to believe that a Communist conspiracy existed at the highest levels of the US government.

To support quality improvement initiatives, the adult-gerontology clinical nurse specialist conducts a root cause analysis utilizing a Pareto chart for identifying the:

The adult-gerontology clinical nurse specialist presents a research study on the cardiovascular risk factors of patients who are over age 50. It is noted that all study participants are between 50 and 60 years of age. The potential bias in the study is due to the:

Which drug should be avoided during the general anesthetic of a patient undergoing a posterior spinal fusion if the surgeon will be using electromyography during the surgical procedure? 

During ocular alignment testing you note that the left eye drifts upward at rest.  This would be indicative of which type of lesion?

In assessing a patient’s MAJOR RISK factors for heart disease, which most important assessment questions would the nurse want to include when taking a history?

A high-volume, procedural-based unit experiences significant patient wait times. Leading the team in a continuous quality improvement analysis, the nurse executive begins by constructing a:

What was the battle cry of the Meiji during the Meiji restoration?