Two proteins elute in fractions 30 and 40 of a size exclusion column on their own. You mix them in equal molar ratios and run them over the same column, and you only see peaks in fractions 30 and 40. What would you conclude?
What is the purpose of adding EDTA to a lysate?
What is the purpose of adding EDTA to a lysate?
When expressing proteins such as insulin, yeast may be more…
When expressing proteins such as insulin, yeast may be more advantageous than E. coli because
A technique that is useful in identifying RNA-protein intera…
A technique that is useful in identifying RNA-protein interactions is
Which of the following is TRUE regarding purifying proteins…
Which of the following is TRUE regarding purifying proteins using the ammonium sulfate precipitation?
Why does the phage vector system have lower levels of backgr…
Why does the phage vector system have lower levels of background expression than the Ptac system?
You purify 100 ml of protein, and the buffer has 200 mM NaCl…
You purify 100 ml of protein, and the buffer has 200 mM NaCl. How many dialyses will you need to perform to reduce the salt concentration to below 10 mM, if dialyzing into 1 L of buffer with no salt?
Which of the following techniques has an identical setup to…
Which of the following techniques has an identical setup to a western blot?
The native gel below shows three proteins (A-C) run alone, a…
The native gel below shows three proteins (A-C) run alone, and then with a protein of interest (P). Which protein interacts with protein P?
In which expression system does T7 RNA polymerase enzyme tra…
In which expression system does T7 RNA polymerase enzyme transcribe the gene of interest on the plasmid into mRNA?