A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following sequence:  5’-GGATCCATGACTAGTCCGAC-3’. A segment of DNA is cloned into a vector and then the vector DNA is denatured and subjected to dideoxy DNA sequencing method. Below is the DNA sequence from a region of the vector. The primer-annealing site is shown in bold and underlined.  3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACT-5’   Based on the sequence above, what would be the size(s) of the band(s) (i.e., the number of nucleotides in each band) in which dideoxyT had been added to the sequencing reaction?

Metagenomics is:

The identification of a stop codon for a particular gene is an example of

In the biotechnological production of human insulin (Humulin), how are the A or B chains separated from beta-galactosidase?

You can use tandem mass spectrometry to:

The restriction enzymes shown below cut DNA in the following manner. The slashes indicate where cutting occurs. BamHI                         G\GATCC                                     CCTAG\G   EcoRI                          G\AATTC                                     CTTAA\G        HaeII                           GGCGC\C                                    C\CGCGG   PstI                             CTGCA\G                                    G\ACGTC Which of these enzymes would generate sticky ends that would be the least stable when they bind to complementary sticky ends?

Hematopoietic stem cells (blood cells) in your body are

Trinucleotide repeat expansion is caused by

When a fragment of chromosomal DNA is inserted into a vector, how many covalent bonds are made via DNA ligase? 

Work in a forensic lab and you need to do a paternity test. You would: