You are in a lab that specializes in microscopy and you are studying breast cancer. Which microscopy technique would you use to perform the following experiments? For each experiment select the best possible technique from the drop-down menu. Use each technique only once. 

Proteins run on SDS-PAGE gels are separated based on their size (mass) because SDS maintains intrinsic charge and beta-mercaptoethanol preserves three-dimensional structure.

There are three exams each worth 75 points.  Together these three exams count for _________ of my grade.

A Positron Emission Tomography (PET) scan visualizes brain activity by imaging energy use. Why does energy use correlate with neural activity?

Cut-and-Run is a technique used to identify genomic DNA sequences bound by a particular protein.

Ion channels are lined with amino acids that create a selectivity filter, allowing only certain ions to pass based on their size and charge.

Caribou are adapted to live in the Arctic, which means their cells are functional in extreme cold. Based on your knowledge of cell membranes, which prediction would you make about the lipid bilayer of caribou cells?

For each of the following sentences, fill in the blanks with the best word or phrase selected from the provided lists. Not all words or phrases will be used; each word or phrase should be used only once. AbsorptionCondenser Embedding GFP fusion protein GFP reporter line FixationFluorescent moleculeIntensity MagnificationMicrotome Numerical Aperture (N.A.)ResolutionSlicerStainingWavelength

If a Ras GTPase mutant is locked in an activated state (as often occurs in cancer), is there more likely a loss-of-function mutation in the GEF or the GAP?

Use the drop down menu to select the best word or phrase matching each description, or matching the colored structure in each image. Not all words or phrases will be used; each word or phrase will match only one image or description.   A GTPase that forms a spiral around the neck of a budding clathrin-coated vesicle, constricting to allow membrane fusion and vesicle release. Coiled-coil proteins that form long tethers with Rab binding sites, these are distinct on each Golgi stack to capture different vesicles.