A restriction enzyme binds to the restriction site shown her…

A restriction enzyme binds to the restriction site shown here, cutting between adjacent thymine and cytosine nucleotides. 5′ ………..TCGCGA………….3′3′ ………..AGCGCT………….5′ Which of the following pairs of sequences indicates the sticky ends that are formed by this restriction enzyme?

12. What are the 3 main assumptions of OLS as expressed in v…

12. What are the 3 main assumptions of OLS as expressed in video lectures and in your textbook? For full credit please discuss each in full detail. Can we say that the OLS estimator is BLUE if these thee assumptions hold? Explain why or why not. Discuss what BLUE stands for.

A good question is testable and uses what is known about the…

A good question is testable and uses what is known about the experimental organism and the concept investigated to test new ideas about the factors that can result in a change. A good question will always be rooted in prior scientific knowledge, often relating to the evolutionary history of the organism or process. An example of a good question is, “How does temperature affect the rate of cellular respiration?” Because we know that molecules move more rapidly as temperature increases, we can predict that the rate of reactions involved in cellular respiration will increase. An example of a poor question is, “Does the type of music played affect the rate of cellular respiration in germinating peas?” This is because there is no evolutionary reason that peas might respond to say, classical music versus rock music played at the same volume. Using this information as a guide, which of the following would be the most valid scientific question to pose considering the evolutionary history of peas?

The figure below is a map of the plasmid pGEN 101. The plasm…

The figure below is a map of the plasmid pGEN 101. The plasmid is 20 kb, which stands for kilobases, or thousands of base pairs. It shows the restriction sites for two different enzymes–BamHI and EcoRI–and the resulting fragment sizes. Study this figure to understand what this means. You can use the results of electrophoresis to determine which restriction enzyme was incubated with the plasmid DNA. There is only one restriction site for the enzyme EcoRI. Imagine the result of mixing this plasmid with EcoRI. The circular plasmid would be cut open, resulting in a single large linear fragment of DNA that would be 20 kb in size. Lane 2 shows a piece of DNA that is 20 kb and so must have been digested with EcoRI. Which lane shows a digest with only the restriction enzyme BamHI?