You have purified a new peptide hormone. To determine its amino acid sequence you have digested the polypeptide with trypsin and in a separate reaction you have cleaved the polypeptide with cyanogen bromide. Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following. Ser─Leu Asp─Val─Arg Val─Met─Glu─Lys Ser─Gln─Met─His─Lys Ile─Phe─Met─Leu─Cys─Arg Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation: His─Lys─Ser─Leu Asp─Val─Arg─Val─Met Glu─Lys─Ile─Phe─Met Leu─Cys─Arg─Ser─Gln─Met Determine the sequence of the intact protein.
When calculating days away from work only count business or…
When calculating days away from work only count business or scheduled working days.
The general duty clause is typically cited for violations fo…
The general duty clause is typically cited for violations for which an OSHA standard currently exists, but for which the standard is not strict enough (e.g., the standard is NOT protective enough and/or the penalty is too LOW).
It is always a good idea to wear PPE even when engineering c…
It is always a good idea to wear PPE even when engineering controls are in place just to be a little bit safer.
Full compliance with OSHA noise standards will completely el…
Full compliance with OSHA noise standards will completely eliminate hearing loss in the workplace.
While physical and atmospheric hazards are considered for th…
While physical and atmospheric hazards are considered for the determination of whether or not a space is a permit-required confined space, configuration hazards such as sloping walls, were left out of the confined space standard due to pressure from the agriculture industry which felt that this would place an undue economic burden on the industry.
According to the OSHA lockout/tagout standard (1910.147)
According to the OSHA lockout/tagout standard (1910.147)
This unguarded shaft is…
This unguarded shaft is…
The inner ear responds differently to various frequencies (s…
The inner ear responds differently to various frequencies (some frequencies sound louder and some quieter).
Noise is unwanted sound that can be irritating and damaging.
Noise is unwanted sound that can be irritating and damaging.