Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA haplotypes of the donor and recipient at the highest resolution?

A child has disputed paternity. The child has the following STR haplotype: TPOX = 7/8; D5S818 = 11/12; D13S317 = 9/10; vWA = 11/12; D21S11 = 27/28. Alleged Father 1 has the following STR haplotype: TPOX = 6/10; D5S818 = 9/13; D13S317 = 9/14; vWA = 14/16; D21S11 = 30/33. What can be said of Alleged Father 1?

Which of the following controls can distinguish between a true-negative in a nucleic acid amplification assay from a false-negative in which amplification failed in the specimen?

A patient is assessed for B-cell clonality by examining the immunoglobulin heavy chain gene rearrangements by PCR using primers complementary to the FR3 and joining regions of the immunoglobulin heavy chain gene. When the products are resolved by gel electrophoresis, the patient lane has a smear and does not have a sharp band. Controls were all acceptable. This result is interpreted as which of the following?

Which of the following organizations has established standards used by laboratory professionals when developing, implementing, and performing molecular-based laboratory tests?

A patient has been diagnosed with breast cancer, and her physician wants to know if her tumor cells have a TP53 mutation. Which of the following laboratory methods is performed most often and will give the best information regarding whether TP53 mutations are present in her tumor cells?

Whole blood was sent to the laboratory for viral load determination of HIV (an RNA virus). It was received on a Saturday morning, and the technologist working in the receiving section of the laboratory put the tube in the refrigerator at 2o-8oC. The molecular technologist came on Monday morning and collected the specimens that had been sent to the laboratory over the weekend. How should this sample be handled on Monday morning?

The purpose of endonucleases is to:

In inorganic DNA isolation or “salting out” procedures, in the presence of low pH and high salt concentrations, which intracellular component precipitates out of solution?

An outbreak of Salmonella has occurred in the community. Molecular-based assays need to be performed in order to determine if all the Salmonella isolated at hospital X are related to each other and part of the outbreak. Laboratory technologists have accumulated 10 different cultures growing Salmonella and suspect that there may be more isolated in the next few days. The supervisor has asked that the molecular-based assays be performed on as many of the isolates as possible. How should these organisms be handled until the molecular-based assays can be performed in about 3 days?