Next generation sequencing, like Illumina

Being able to fluorescently label terminating nucleotides is advantageous because

Shotgun sequencing allows for pairing of repeating/overlapping sequence segments of DNA fragments to piece together a longer sequence than otherwise possible  

Nanopores allow for DNA sequence detection without terminating nucleotides  

Genetic mapping involves:

In the early Puritan and Quaker colonies why were the punishments overwhelmingly punitive and repressive?

What did John Howard feel needed to be in place for meaningful prison reform to occur?

Was there an emphasis on rehabilitation in the early colonies?

When coded in a WHERE clause, which of the following search conditions will NOT return a result set that includes all invoices with an invoice_total value of $2117.33 or less?

A patient has been referred to outpatient physical therapy for right upper extremity lymphedema management. She is status post right mastectomy with lymph node removal. She has no significant past medical history.   QUESTION: Which of the following would not be appropriate in therapy management of her lymphedema?