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Tо elucidаte the rоle оf estrаdiol feedbаck on GnRH pulse generation in postpartum beef cows and its implications for the resumption of estrous cyclicity. Objective 1: To quantify changes in GnRH pulse frequency and amplitude in response to varying levels of estradiol during the postpartum period. Hypothesis 1: Estradiol levels positively regulate GnRH pulse frequency and amplitude, facilitating the resumption of estrous cyclicity postpartum. Objective 2: To investigate the relationship between estradiol levels and LH surge onset in postpartum cows. Hypothesis 2: A critical threshold of estradiol is required to trigger the preovulatory LH surge, with delayed estradiol rises contributing to anovulation in early postpartum cows. Objective 3: To assess the impact of estradiol supplementation on the timing of the first postpartum ovulation. Hypothesis 3: Estradiol supplementation during the early postpartum period will advance the timing of first ovulation by enhancing GnRH and LH surge dynamics. References: Stevenson, J.S., et al. (1999). Estradiol Regulation of Gonadotropin Secretion in Postpartum Beef Cows. Journal of Animal Science, 77(5), 1320–1326. Wiltbank, M.C., et al. (2018). Regulation of Estradiol and LH Surge in Cows. Theriogenology, 112, 6–15.