I decide to sequence a short piece of DNA that I am interest…
I decide to sequence a short piece of DNA that I am interested in. I used the Sanger sequencing method and my resulting gel looks like this. What is the sequence of my gene? Image Description The above diagram is a square with four columns: A, C, G, and T. As you start at the top of the diagram at 3′ and move down toward 5′, a line in any column indicates that nucleotide occurs in that order of the gene sequence. For example, the nucleotide at the very top is in the A column, and the second nucleotide is in the T column. The following table simulates the above diagram; an X indicates that nucleotide is present in that position. Note to screen reader users: NVDA is recommended for navigating this table. Nucleotide A C G T 3′ (−) X X X X X X X X 5′ (+) X