Level 3 – Chromatography – Columns vs TLC TLC plates are ess…
Level 3 – Chromatography – Columns vs TLC TLC plates are essentially miniature column chromatography experiments. The order in which the analytes travel is proportional to the order in which the analytes would elute off of a column. Four compounds consisting of propanol, cyclohexene, 3-amino-hexanoic acid, and acetic acid, were combined and added onto a silica gel column with a mobile phase of hexane:ethyl acetate (10:1 v/v). Predict the compounds elution order from first to elute (1) to last to elute (4) based on IMFs.