Materials: Three clean test tubes Four beakers Two 5 mL…
Materials: Three clean test tubes Four beakers Two 5 mL Graduated pipet Hydrogen peroxide (H2O2) Catalase (potato) Ruler Wax pencil Procedure: In order to test enzyme concentration, we will be diluting the catalase with water. Use the beakers to dilute the catalase. Mix according to the table below. Beaker Water Catalase Concentration 1 3 mL of water 1 mL of Catalase 25% 2 2 mL of water 2 mL of Catalase 50% 3 1 mL of water 3 mL of Catalase 75% 4 0 mL of water 4 mL of Catalase 100% Label each of your test tubes (E1, E2, E3, and E4). To each of your test tubes add 4 mL of hydrogen peroxide. To E1, add 1 mL of 25% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. To E2, add 1 mL of 50% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. To E3, add 1 mL of 75% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. To E4, add 1 mL of 100% Catalase. Carefully swirl the mix, wait 30 seconds, and record the bubble height (mm) in Table 3. Results: (Watch the Lab 7. Enzymes video) Table 3: Effects of Enzyme Concentration (Copy and paste the table to answer Question 18 and fill it in with your data) Test Tube Catalase Concentration Bubble Height (mm) E1 E2 E3 E4