Spleen cells are taken from a mouse immunized with the LCM v…
Spleen cells are taken from a mouse immunized with the LCM virus. 2 assays are used to determine antigen-specific functional activity of the spleen cells. Assay 1: the spleen cells (T cells) are incubated with macrophages that have been briefly exposed to LCM virus (phagocytized the virus), production of IL-2 by the T cells is considered a positive response. Assay 2: the spleen cells are incubated with LCM-infected target cells, lysis of the infected target cells is considered a positive response. The functional activity of which MHC molecules is detected in Assay 1?