Step 1: Determine if endocannabinoids bind to the receptor(s…
Step 1: Determine if endocannabinoids bind to the receptor(s) in the GPR/TRPV signaling pathway. Before exploring potential cancer treatments, we will explore how endocannabinoids affect the GPR/TRPV signaling pathway. We’ll start by determining whether endocannabinoids bind to the receptor(s) in the pathway and, if so, the impact of this binding on the activity of the receptors GPR and TRPV. The following experiment was conducted. Ten genetically identical cells were collected from healthy breast tissue. The relative activity (% of observed max) of the receptors GPR and TRPV were separately measured in these healthy cells. Then, the cells were exposed to an endocannabinoid suspected to be associated with causing breast cancer (called ENDO1), and the measurements were repeated. Using this information, answer the following questions.