The lymphatic ducts feed into the subclavian veins. 

Questions

The lymphаtic ducts feed intо the subclаviаn veins. 

In the fоllоwing prоcedure, the protein of interest, CA, is initiаlly concentrаted by precipitаtion from a 30% saturated ammonium sulfate solution. If you were interested in using ammonium sulfate precipitation to isolate a highly charged peptide, would you expect the peptide to precipitate from a low or high concentration ammonium sulfate in solution?  Briefly justify your answer.   Procedure from the reference cited below: "Briefly, cells expressing the wild-type CA protein were lysedthrough a microfluidizer. Soluble CA protein in the clarifiedlysate was concentrated by precipitation from 30% saturatedammonium sulfate. CA protein was redissolved in 50 mM Tris(pH 8.0) and functionally purified by the addition of sodiumchloride to a final concentration of 2.5 M. After two rounds offunctional purification, the wild-type CA protein wasresuspended in 50 mM sodium phosphate buffer (pH 7.5)and dialyzed against the same buffer. The dialyzed sample wasfurther purified by a subtractive anion exchange chromatography step using a Q-HP HiTrap column (catalog no. 17-1154-01, GE Healthcare, Piscataway, NJ). Purification of 2Mut and 4Mut CA mutants followed thesame protocol that was used for wild-type CA except that200 mM β-mercaptoethanol was included in all buffersthroughout the process. The functionally purified capsidmutant proteins were redissolved in 50 mM Tris (pH 7.5) and40 mM β-mercaptoethanol prior to dialysis in the same buffer,and a subsequent purification with subtractive anion exchangechromatography was performed." A Trimer of Dimers Is the Basic Building Block for Human Immunodeficiency Virus-1 Capsid Assembly.