Treatment of a polypeptide with cyanogen bromide (chemically…
Treatment of a polypeptide with cyanogen bromide (chemically selectively cleaves peptide bonds on the carboxy side of Met residues) followed by Edman degradation of each of these fragments, gave these four fragment sequences. 1. Asp-Ile-Lys-Gln-Met, 2. Lys, 3. Lys-Phe-Ala-Met, 4. Tyr-Arg-Gly-Met Trypsin hydrolysis of the same polypeptide followed by Edman degradation of each fragment gave these four sequences. 1. Gln-Met-Lys, 2. Gly-Met-Asp-Ile-Lys, 3. Phe-Ala-Met-Lys, 4. Tyr-Arg Based on this information, write out the sequence of the peptide.