Two enzymes of unknown concentrations, A and B, act on the s…
Two enzymes of unknown concentrations, A and B, act on the same substrate, S. The initial velocity (V0) of the reaction catalyzed by Enzyme B is 60% of the V0 for Enzyme A when both enzymes are assayed at = 100 mM. Enzyme A has a Km of 20 mM, and enzyme B has a Km of 100 mM. Both Enzyme A and B have a Vmax of 120 mM/s. (6 pts) A) What is the initial velocity (V0) for Enzyme A with its kinetic parameters and provided? (3 pts) B) From these results, can you draw the conclusion on whether Enzyme A’s kcat is higher/lower than or equal to that of Enzyme B? Explain. (3 pts)