Use this information to answer the next three questions. In…
Use this information to answer the next three questions. In 1966, Andre Jagendorf and Ernest Uribe published a paper on experiments to study hydrogen ion concentrations and ATP production during photosynthesis. The investigators isolated chloroplasts from spinach leaves and disrupted them so that chemical reagents added during the experiment would have access to the thylakoids. These broken chloroplasts were soaked in a “first solution” hydrogen ion concentration and then transferred to a fresh “second solution” of hydrogen ion concentration to create a hydrogen ion concentration gradient across the thylakoid membrane. This second solution also contained ADP and inorganic phosphate. The rate of ATP production was measured from the second solution. This table summarizes Jagendorf and Uribe’s results. Hydrogen Ion Concentration (nmol/liter) Trial # First Solution Second Solution Rate of ATP synthesis 1 10,000 5.0 4 nmol/mg 2 25,000 5.0 11 nmol/mg 3 159,000 5.0 28 nmol/mg 4 63.0 63.0 0 nmol/mg 5 10,000 63.0 0 nmol/mg 6 25,000 63.0 0.5 nmol/mg 7 159,000 63.0 8 nmol/mg