Your company has hired you to analyze different vascular gra…
Your company has hired you to analyze different vascular graft surfaces. A. (1 pts) In your first experiment, you’ve quantified the number of adhered platelets on the different vascular graft surfaces (ePTFE, POC, and POC with heparin incorporated) after a parallel flow chamber assay. Based on the plot below, which surface(s) would be best and which would be worst for a vascular graft? Briefly describe your rationale (2 sentences or less) B. (.5 pts) Do you think outside-in or inside-out integrin activation is likely for your grafts? Briefly (2 sentences or less) describe why. C. (.25 pts) You’re interested in determining which integrin receptor is responsible for platelet adhesion to your vascular grafts for your ePTFE and POC grafts. You’ve found there is an increase in Integrin α11/β1 for both. Which type or integrin receptor is involved based on the graphical representation below? D. (.5 pts) Being a biomedical engineer, you also know that it is very important for your endothelial cells to retain their elastic properties when exposed to a vascular graft. You’ve performed Real-time PCR and quantified Elastin expression in endothelial cells cultured on your grafts after 3 days (ePTFe = orange, POC = green, POC-heparin = blue). You’ve also included the red as a control – is your red a positive or negative control? Provide your rationale – consider if it has higher or lower Elastin expression than your experimental groups. E. (.5 pts) What does real-time PCR quantify (1 sentence or less)?