A plasmid is digested with the enzyme SmaI. On the agarose g…
A plasmid is digested with the enzyme SmaI. On the agarose gel electrophoresis, you observe 2 bands: 250 and 300 bp. A. How many SmaI sites are present in this plasmid? (1 pt) B. What is the size of the plasmid? (1 pt) C. Now you cut the original (undigested by SmaI) plasmid with HindIII. The digestion yields 2 pieces, 50 and “X”. What is the “X” in base pairs? (1 pt)