After performing a 10-fold serial dilution of a viral stock,…
After performing a 10-fold serial dilution of a viral stock, 0.1 mL from each dilution is plated and incubated until plaques form. Using the provided plaque counts, identify the dilution that yields a countable plate (10–100 plaques) and calculate the viral titer (PFU/mL) of the original stock. Show your work. Dilution # of plaques Volume Plated (mL) PFU/mL 10-2 TNTC 0.1 ? 10-3 210 0.1 ? 10-4 126 0.1 ? 10-5 42 0.1 ? 10-6 6 0.1 ?