The figure below is from Vandputte, et al., Applied and Envi…

The figure below is from Vandputte, et al., Applied and Environmental Microbiology, January 2010, Vol. 76: p. 243-253 (no need to look at the paper). They used Chromobacterium violaceum (A) and Pseudomonas aeruginosa (B) to examine the effects of the leaf and bark extracts (extracted using DMSO) from a tree (Combretum albiflorum). All of the wells have acyl homoserine lactones added and each row has three wells with the same samples (triplicate). The blue color in A is due to production of violacein, a pigment. The red color in B is due to pyocyanin production, a substance that is toxic to eukaryotic cells. The *** indicates this bar is significantly different from the DMSO (solvent control) bar. Question: What kind of system seems to be regulating the production of these colored substances? How does this system work? Why would they measure not only the production of the colored substances but also bacterial growth? What statement can you make regarding this figure? (What can you say about the leaf and bark extracts and which you would most prefer for what and why?)    

Archie is a 2-year old boy who is being evaluated for occupa…

Archie is a 2-year old boy who is being evaluated for occupational therapy services through early intervention. He has a diagnosis of Trisomy 21. The state he lives in does not require a specific assessment battery to be used, but testing must includeALL 5 domains related to function (motor, cognition, communication, adaptive skills, social-emotional). Which of the following would make sense to use with Archie to determine if he is eligible for early intervention services?