You enroll 12 laboratory-bred 2 year old Beagles in a study…
You enroll 12 laboratory-bred 2 year old Beagles in a study analyzing the nephrotoxicity of a new drug. 6 were randomly assigned to receive the experimental treatment, while the other 6 receive the control treatment. You measure the effects of each treatment on urine protein-creatine ratio (UPC) after 30 days of enrollment. After analyzing the results, you find that the experimental treatment is significantly less nephrotoxic than the control (p < 0.05). What is the major limitation of this study?